A Protein, VPg, Covalently Linked to 36S Calicivirus RNA

J Gen Virol. 1980 Mar;47(1):215-20. doi: 10.1099/0022-1317-47-1-215.

Abstract

Proteins associated with 36S virus RNA from Vero cells infected with San Miguel sea lion virus, type 2 (SMSV-2), were labelled with 125I. One protein, VPg, remained linked to RNA when subjected to deproteinization techniques. VPg labelled wtih 32P was observed on 36S RNA from purified virions; the quantity of label was compatible with two phosphates per genome. The estimated mol. wt. of SMSV-2 VPg was 15 000.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Caliciviridae / analysis*
  • Cells, Cultured
  • Chemical Fractionation
  • Electrophoresis, Polyacrylamide Gel
  • Molecular Weight
  • Nucleosides / isolation & purification
  • Protein Denaturation
  • RNA, Viral / analysis*
  • Seals, Earless
  • Viral Proteins / analysis*
  • Virus Replication

Substances

  • Nucleosides
  • RNA, Viral
  • Viral Proteins