Autoradiographic evidence for hepatic lobular concentration gradient of bile acid derivative

Am J Physiol. 1980 Mar;238(3):G233-7. doi: 10.1152/ajpgi.1980.238.3.G233.


Using an iodinated bile-acid analog with hepatic uptake and transport characteristics similar to conventional bile acids, the hepatic lobular gradient concept of Goresky was studied utilizing autoradiography. 125I-labeled cholylglycylhistamine (125I-CGH) was infused into the portal veins of male Sprague-Dawley rats and the livers were fixed for light microscopic autoradiography at 1 and 5 min after infusion. In two animals, sequential samples of bile were collected to assess the transport characteristics of 125I-CGH. By 1 min, virtually all (98%) of the injected 125I-CGH was taken up and retained by hepatocytes after perfusion fixation. Less than 15% of the label was lost during subsequent tissue processing. 125I-CGH appeared in bile within minutes, reaching maximum levels at 7 min. Quantitative autoradiography demonstrated that the first six to nine periportal hepatocytes were, by far, the most active (P less than 0.0005) in sequestering the bile-acid analog than were the remaining cells in the lobule. This study, therefore, provides the first autoradiographic evidence of a hepatic lobular concentration gradient for the uptake of a bile-acid analog.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Bile / metabolism
  • Biological Transport
  • Fasting
  • Glycocholic Acid / analogs & derivatives*
  • Glycocholic Acid / metabolism
  • Histamine / analogs & derivatives*
  • Histamine / metabolism
  • Infusions, Parenteral
  • Liver / metabolism*
  • Male
  • Rats


  • cholylglycylhistamine
  • Histamine
  • Glycocholic Acid