Restricted subpopulations of DNA antibodies in kidneys of mice with systemic lupus. Comparison of antibodies in serum and renal eluates

Arthritis Rheum. 1980 Apr;23(4):392-403. doi: 10.1002/art.1780230402.


Sera from MRL/1, BXSB, and NZB/NZW mice, which develop IgG antibodies to DNA and glomerular deposits of DNA-antiDNA immune complexes, were studied by isoelectric focusing. A large array of IgG antibodies with isoelectric points ranging from pH 5.5--9.0 were found to bind double-stranded DNA. Antibodies with isoelectric points from 8.0--8.5 were significantly more frequent than antibodies focusing in all other pH ranges. In contrast, glomerular eluates from MRL/1 and NZB/NZW mice contained a restricted number of DNA-binding bands, all of which focused at pH 8.0--9.0. Anti-DNA with isoelectric points from pH 8.0--9.0 may be more pathogenic for the kidney than other subpopulations.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies / isolation & purification*
  • Antigen-Antibody Complex
  • DNA / immunology*
  • Female
  • Immunoglobulin G / immunology
  • Immunoglobulin G / isolation & purification
  • Isoelectric Focusing
  • Kidney / immunology
  • Kidney / physiopathology
  • Lupus Erythematosus, Systemic / genetics*
  • Lupus Erythematosus, Systemic / immunology
  • Male
  • Mice / genetics*
  • Mice / immunology
  • Proteinuria / immunology


  • Antibodies
  • Antigen-Antibody Complex
  • Immunoglobulin G
  • DNA