Abstract
The results of dimethyl suberimidate cross-linking experiments demonstrate that histone H1 can be cross-linked quantitatively to the histone octamer of mononucleosomes to yield a histone nonamer. By combining our cross-linking procedure with two-dimensional electrophoretic techniques, we show further that it is possible to trace the nucleosomal origins of a spectrum of cross-linked chromatin products.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cattle
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Cell Nucleus / analysis
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Chromatin / analysis*
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Cross-Linking Reagents
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Dimethyl Suberimidate
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Electrophoresis, Polyacrylamide Gel
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Histones / analysis*
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Macromolecular Substances
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Molecular Weight
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Thymus Gland / analysis
Substances
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Chromatin
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Cross-Linking Reagents
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Histones
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Macromolecular Substances
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Dimethyl Suberimidate