Catalysis of adenovirus DNA synthesis in vitro by DNA polymerase gamma

J Gen Virol. 1980 May;48(1):231-6. doi: 10.1099/0022-1317-48-1-231.


DNA replication complexes were purified from adenovirus type 5 (Ad5)-infected HeLa cells. DNA synthesis by these complexes in vitro was extremely sensitive to the inhibitors dideoxythymidine triphosphate, N-ethyl maleimide and p-hydroxymercuribenzoate. The bound DNA polymerase was released from the complexes by limited digestion with micrococcal nuclease. This released polymerase preferred poly(rA):(dT)12-18 as template over activated calf thymus DNA. These results are compatible with the major polymerase in the replication complex being of the gamma class.

MeSH terms

  • Adenoviridae / metabolism*
  • DNA, Viral / biosynthesis*
  • DNA-Directed DNA Polymerase / metabolism*
  • Dideoxynucleotides
  • Ethylmaleimide / pharmacology
  • Hydroxymercuribenzoates / pharmacology
  • Replicon*
  • Templates, Genetic
  • Thymine Nucleotides / pharmacology


  • DNA, Viral
  • Dideoxynucleotides
  • Hydroxymercuribenzoates
  • Thymine Nucleotides
  • DNA-Directed DNA Polymerase
  • 2',3'-dideoxythymidine triphosphate
  • Ethylmaleimide