Cell Cycle-Dependent Replication of the DNA of Minute Virus of Mice, a Parvovirus

Biochim Biophys Acta. 1980 May 30;607(3):420-31. doi: 10.1016/0005-2787(80)90152-5.


The formation of double-stranded viral DNA was examined in synchronized cells infected with minute virus of mice in early G1 phase. In the infected cells, a minimum of 50-100 copies of the input single-stranded DNA have been converted to a double-stranded form by mid S phase. In well-synchronized cells, the amount of double-stranded form by mid A phase. In well-synchronized cells, the amount of double-stranded viral DNA detected during G1 is on the order of a few copies per cell or less. When cells are infected in the presence of the thymidine analog, 5-bromodeoxyuridine, viral DNA synthesis is inhibited. However, 5-bromodeoxyuridine does not inhibit host DNA synthesis nor does it prevent replication of viral DNA if added to the infected cells in late S phase. Viral DNA replication first becomes resistant to 5-bromodeoxyuridine inhibition at the beginning of S phase. As 5-bromodeoxyuridine appears to specifically block early steps in viral DNA synthesis but not the subsequent replication of the DNA, the conversion of the input viral genome to a double-stranded form which undergoes further replication appears to be a S phase-specific event.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bromodeoxyuridine / pharmacology
  • Cell Cycle*
  • Cells, Cultured
  • DNA Replication* / drug effects
  • DNA, Single-Stranded / metabolism
  • Guinea Pigs
  • Interphase*
  • Minute Virus of Mice / metabolism*
  • Parvoviridae / metabolism*
  • Virus Replication* / drug effects


  • DNA, Single-Stranded
  • Bromodeoxyuridine