Prostaglandin production by intact isolated gastric parietal cells

Eur J Pharmacol. 1980 Aug 22;66(1):145-8. doi: 10.1016/0014-2999(80)90309-x.


An enriched population of isolated gastric parietal cells was obtained from canine gastric mucosa. Parietal cells incubated with [14C]arachidonic acid produced radiolabelled PGF2 alpha, PGE2, PGD2 and 6-keto PGF1 alpha (acid hydrolysis product of PGI2). The prostaglandin synthesis was inhibited by indomethacin. Prostaglandin production, as measured by gas chromatography-mass spectrometry demonstrated that PGF2 alpha was produced in the highest quantity followed by PGE2 and 6-keto PGF 1 alpha. This demonstrates that isolated prietal cells are capable of producing prostaglandins. Since prostaglandins have a potent effect on gastric acid secretion, local prostaglandin synthesis could modulate parietal cell function.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arachidonic Acids / metabolism
  • Dogs
  • Gastric Mucosa / cytology
  • Gastric Mucosa / metabolism*
  • Indomethacin / pharmacology
  • Prostaglandins / biosynthesis*


  • Arachidonic Acids
  • Prostaglandins
  • Indomethacin