Separation and quantitation of glycine and taurine conjugates of commonly occurring bile acids in bile, i.e. lithocholic, deoxycholic, chenodeoxycholic, ursodeoxycholic and cholic acids in their naturally occurring states have been successfully accomplished using high-performance liquid chromatography. No preliminary purification of bile acids is required except ethanol extraction of bile. A muBondapak C18 column and acetonitrile--methanol--phosphate buffer and ultraviolet detector at 200 nm were used. Detection limit weas 0.05 microgram and linearity was observed in the range up to 16 microgram. Bile acid composition of ten randomly chosen normal human gallbladder bile samples is given. A large difference in bile acid composition between glycine and taurine conjugates was found to be present.