Pyrimidine-specific chemical reactions useful for DNA sequencing

Nucleic Acids Res. 1980 Oct 24;8(20):4613-9. doi: 10.1093/nar/8.20.4613.

Abstract

Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cytosine / analysis
  • DNA*
  • Hydroxylamines
  • Indicators and Reagents
  • Methods
  • Potassium Permanganate
  • Pyrimidines / analysis*
  • Thymidine / analysis

Substances

  • Hydroxylamines
  • Indicators and Reagents
  • Pyrimidines
  • Potassium Permanganate
  • Cytosine
  • DNA
  • Thymidine