Abstract
Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Base Sequence
-
Cytosine / analysis
-
DNA*
-
Hydroxylamines
-
Indicators and Reagents
-
Methods
-
Potassium Permanganate
-
Pyrimidines / analysis*
-
Thymidine / analysis
Substances
-
Hydroxylamines
-
Indicators and Reagents
-
Pyrimidines
-
Potassium Permanganate
-
Cytosine
-
DNA
-
Thymidine