Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates

Nucleic Acids Res. 1980 Mar 11;8(5):1081-96. doi: 10.1093/nar/8.5.1081.

Abstract

A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold better overall yields.

Publication types

  • Comparative Study

MeSH terms

  • Chemical Phenomena
  • Chemistry
  • Methods
  • Oligodeoxyribonucleotides / chemical synthesis*
  • Oligonucleotides / chemical synthesis*
  • Organophosphates
  • Resins, Plant

Substances

  • Oligodeoxyribonucleotides
  • Oligonucleotides
  • Organophosphates
  • Resins, Plant