A hemopoietic cell line dependent upon a factor in pokeweed mitogen-stimulated spleen cell conditioning medium

J Cell Physiol. 1980 Nov;105(2):379-84. doi: 10.1002/jcp.1041050221.

Abstract

A continuously growing cell line (FMP1.1) has been isolated, which is dependent upon a factor in PWCM for both growth and survival. FMP1.1 appears to be a mast cell, since IgE receptors are present and their granules react specifically with a mast cell granule stain. The factor in PWCM may be a glycoprotein and has biochemical properties in common with PWCM factors, which stimulate at least four other lineages of hemopoietic colony-forming cell. This line provides a pure population of cells as a model for studying molecular events of hemopoietic precursor cell growth and differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Division / drug effects
  • Cell Line*
  • Cell Survival / drug effects
  • Culture Media
  • Hematopoiesis*
  • Male
  • Mast Cells / cytology*
  • Mice
  • Mice, Inbred DBA
  • Pokeweed Mitogens / pharmacology*
  • Spleen

Substances

  • Culture Media
  • Pokeweed Mitogens