Six new species of Penicillium Link ex Fries are described and illustrated. Four of them have been recovered from the atmosphere in Madrid, Spain, the other two species were isolated from must and from soil respectively. They clearly differ from all species of the genus described so far and are, therefore described and proposed as new taxa: Penicillium gerundense sp. nov., Penicillium valentinum sp. nov., Penicillium alicantinum sp. nov., Penicillium malacaense sp. nov., Penicillium tarraconense sp. nov., and Penicillium vasconiae sp. nov.