Enzymatic Assay of Serum Sialic Acid

Clin Chim Acta. 1980 Dec 22;108(3):493-8. doi: 10.1016/0009-8981(80)90360-5.

Abstract

Serum sialic acid has been assayed enzymatically. The reaction includes neuraminidase hydrolysis of glycoprotein, cleavage of sialic acid to pyruvate by N-acetyl neuraminic acid (NANA)-aldolase, oxidation of pyruvate by pyruvate oxidase which produces hydrogen peroxide, and colorimetry of hydrogen peroxide using the peroxidase-p-chlorophenol-4-amino-antipyrine method. This method showed good correlation between a chemical method (r = 0.984), good recovery (98.8%) and good reproducibility (within-run-precision: 1.0% C.V.; day-to-day precision: 1.9% C.V). Intrinsic serum pyruvate produces an equimolar positive effect. The normal value range is 1.94 +/- 0.29 mmol/l (mean +/- S.D.,n = 24).

MeSH terms

  • Chemical Phenomena
  • Chemistry
  • Female
  • Humans
  • Male
  • Methods
  • Neuraminidase
  • Oxo-Acid-Lyases
  • Reference Values
  • Sialic Acids / blood*

Substances

  • Sialic Acids
  • Neuraminidase
  • Oxo-Acid-Lyases
  • N-acetylneuraminate lyase