Developmental and regional expression pattern of a novel NMDA receptor-like subunit (NMDAR-L) in the rodent brain

J Neurosci. 1995 Oct;15(10):6509-20. doi: 10.1523/JNEUROSCI.15-10-06509.1995.


A novel NMDA receptor-like (NMDAR-L) cDNA was isolated that contained an open reading frame coding for a predicted polypeptide of 1115 amino acids that shares approximately 27% identity with NMDA receptor subunits. In situ hybridization experiments indicated that NMDAR-L mRNA was expressed in the developing rodent CNS. On postnatal day 1 (P1), NMDAR-L mRNA expression was pronounced in the entorhinal cortex, the subiculum and the thalamus, in layer V of the developing neocortex, in the superior and inferior colliculi, and various regions of the hindbrain, excluding the cerebellum. On P5, NMDAR-L mRNA was expressed in layer V of the neocortex, in the entorhinal cortex, in the subiculum, and in the thalamus. On P14, NMDAR-L mRNA was expressed in layers II-VI of the neocortex, in the entorhinal and piriform cortex, in the subiculum and CA1 field, and in the nucleus of the lateral olfactory tract. In the adult brain, NMDAR-L mRNA was detected predominately in the nucleus of the lateral olfactory tract. Injection of NMDAR-L cRNA into Xenopus oocytes did not lead to the expression of homomeric glutamate-activated channels. However, coinjection of the triple combination of NMDAR-L with NMDAR1 and NMDAR2B cRNAs led to a striking decrease in the current magnitude compared to currents obtained after coexpression of the double combination of NMDAR1 with NMDAR2B. While the function of NMDAR-L remains to be established, its developmental and regional expression pattern suggests that NMDAR-L may influence axonal outgrowth and synaptogenesis during brain development.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / metabolism*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism*
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Molecular Probes / genetics
  • Molecular Sequence Data
  • Oocytes / metabolism
  • Polymerase Chain Reaction
  • RNA, Complementary / metabolism
  • RNA, Messenger / metabolism
  • Rats
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Rodentia / metabolism*
  • Tissue Distribution
  • Xenopus


  • Grin3a protein, rat
  • Membrane Glycoproteins
  • Molecular Probes
  • RNA, Complementary
  • RNA, Messenger
  • Receptors, N-Methyl-D-Aspartate

Associated data

  • GENBANK/U29873