Effect of high-affinity anti-Pseudomonas aeruginosa lipopolysaccharide antibodies induced by immunization on the rate of Pseudomonas aeruginosa infection in patients with cystic fibrosis

J Pediatr. 1995 Nov;127(5):711-7. doi: 10.1016/s0022-3476(95)70158-3.


Patients with cystic fibrosis (CF; N = 26) and with no prior history of infection with Pseudomonas aeruginosa were immunized with an octavalent O-polysaccharide-toxin A conjugate vaccine. During the next 4 years, 16 patients (61.5%) remained free of infection and 10 (38.5%) became infected. Total serum antilipopolysaccharide (LPS) antibody levels induced by immunization were comparable in infected and noninfected patients. In contrast, 12 of 16 noninfected versus 3 of 10 infected patients (p = 0.024) mounted and maintained a high-affinity anti-LPS antibody response. When compared retrospectively with the rate in a group of age- and gender-matched, nonimmunized, noncolonized patients with CF, the rate at which P. aeruginosa infections were acquired was significantly lower (p < or = 0.02) among all immunized versus nonimmunized patients during the first 2 years of observation. Subsequently, only those immunized patients who maintained a high-affinity anti-LPS antibody response had a significant reduction (p < or = 0.014) in the rate of infection during years 3 and 4. Smooth, typeable strains of P. aeruginosa predominated among immunized patients; rough, nontypeable strains were most frequently isolated from nonimmunized patients. Mucoid variants were isolated from one immunized patient versus six nonimmunized patients. These results indicate that the induction of a high-affinity P. aeruginosa anti-LPS antibody response can influence the rate of infection in patients with CF.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Bacterial / blood
  • Antibodies, Bacterial / immunology*
  • Antibody Affinity / immunology*
  • Bacterial Vaccines / administration & dosage
  • Bacterial Vaccines / immunology*
  • Child
  • Child, Preschool
  • Cystic Fibrosis / complications
  • Cystic Fibrosis / immunology*
  • Dose-Response Relationship, Immunologic
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Immunization*
  • Infant
  • Lipopolysaccharides / immunology*
  • Male
  • Pseudomonas Infections / etiology
  • Pseudomonas Infections / immunology
  • Pseudomonas Infections / prevention & control*
  • Pseudomonas aeruginosa / immunology*
  • Retrospective Studies
  • Vaccines, Conjugate / administration & dosage
  • Vaccines, Conjugate / immunology


  • Antibodies, Bacterial
  • Bacterial Vaccines
  • Lipopolysaccharides
  • Vaccines, Conjugate