Molecular biology studies on mesothelioma tumor samples: preliminary data on H-ras, p21, and SV40

J Environ Pathol Toxicol Oncol. 1995;14(1):29-34.


The ras gene is one of the oncogenes most commonly detected in human cancers; this protooncogene is converted to active oncogene by point mutations occurring at either codon 12, 13, or 61. SV40 is a DNA-transforming simian virus, a 105 bp sequence of which has been shown recently to be present in a significant fraction of human mesothelioma cells. Eleven human malignant mesotheliomas were examined for H-ras gene mutations at codon 12, 13, and 61 and for the presence of SV40-like sequences. DNA prepared from formalin-fixed and paraffin-embedded tissue was amplified by means of PCR and analyzed using designed restriction fragment length polymorphism. No mutation with respect to H-ras was found in any tumor sample, but the majority of mesothelioma cells contained SV40-like sequences.

MeSH terms

  • Adult
  • Aged
  • Base Sequence
  • DNA, Viral / isolation & purification
  • Female
  • Genes, ras / genetics*
  • Humans
  • Male
  • Mesothelioma / genetics*
  • Mesothelioma / virology*
  • Middle Aged
  • Molecular Sequence Data
  • Mutation / genetics
  • Simian virus 40 / isolation & purification*


  • DNA, Viral