Syngeneic bone marrow transplantation eliminates V beta 8.2 T lymphocytes from the spinal cord of Lewis rats with experimental allergic encephalomyelitis

J Neurosci Res. 1995 Jul 1;41(4):526-31. doi: 10.1002/jnr.490410412.


Experimental allergic encephalomyelitis (EAE), an animal model for multiple sclerosis (MS), is a paralytic disease of the central nervous system (CNS) mediated by T-lymphocytes reactive to myelin basic protein (MBP). Lewis rats actively immunized with fragment 68 to 82 of guinea pig MBP develop a monophasic disease with spontaneous recovery. Lymphocyte recognition of the primary encephalitogenic sequence of MBP (fragment 68 to 82) is V beta 8.2 T cell receptor (TCR) skewed [1-3]. Lewis rats in clinical remission at 1 month and 3 months after spontaneous resolution of EAE retain V beta 8.2 T-lymphocytes in the CNS when analyzed by reverse transcriptase polymerase chain reaction or in situ hybridization. In contrast, 1 and 3 months after clinical remission from syngeneic bone marrow transplantation, V beta 8.2 T lymphocytes are absent from the CNS. During clinically active EAE and inflammatory breakdown of the blood-brain barrier, immune ablation and reconstitution with syngeneic bone marrow results in clinical tolerance of the new immune system to myelin.

MeSH terms

  • Animals
  • Bone Marrow Transplantation*
  • Disease Models, Animal
  • Encephalomyelitis / metabolism*
  • Female
  • Immunization
  • In Situ Hybridization
  • Lymphocytes / metabolism*
  • Multiple Sclerosis
  • Myelin Proteins / metabolism
  • Polymerase Chain Reaction
  • Rats
  • Rats, Inbred Strains
  • Spinal Cord / pathology*
  • T-Lymphocytes


  • Myelin Proteins