Bacillus licheniformis bacitracin-resistance ABC transporter: relationship to mammalian multidrug resistance

Mol Microbiol. 1995 Jun;16(5):969-76. doi: 10.1111/j.1365-2958.1995.tb02322.x.


The nucleotide sequence of the Bacillus licheniformis bacitracin-resistance locus was determined. The presence of three open reading frames, bcrA, bcrB and bcrC, was revealed. The BcrA protein shares a high degree of homology with the hydrophilic ATP-binding components of the ABC family of transport proteins. The bcrB and bcrC genes were found to encode hydrophobic proteins, which may function as membrane components of the permease. Apart from Bacillus subtilis, these genes also confer resistance upon the Gram-negative Escherichia coli. The presumed function of the Bcr transporter is to remove the bacitracin molecule from its membrane target. In addition to the homology of the nucleotide-binding sites, BcrA protein and mammalian multidrug transporter or P-glycoprotein share collateral detergent sensitivity of resistant cells and possibly the mode of Bcr transport activity within the membrane. The advantage of the resistance phenotype of the Bcr transporter was used to construct deletions within the nucleotide-binding protein to determine the importance of various regions in transport.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / metabolism
  • Amino Acid Sequence
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Bacillus / drug effects
  • Bacillus / genetics*
  • Bacillus / metabolism
  • Bacillus subtilis / genetics
  • Bacillus subtilis / metabolism
  • Bacitracin / pharmacology*
  • Conserved Sequence
  • Drug Resistance, Microbial / genetics*
  • Drug Resistance, Multiple / genetics*
  • Escherichia coli / drug effects
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Humans
  • Mammals
  • Molecular Sequence Data
  • Open Reading Frames
  • Restriction Mapping
  • Sequence Homology, Amino Acid


  • ATP-Binding Cassette Transporters
  • Anti-Bacterial Agents
  • Bacitracin

Associated data

  • GENBANK/L20573