Purification of recombinant Rho/Rac/G25K from Escherichia coli

Methods Enzymol. 1995;256:3-10. doi: 10.1016/0076-6879(95)56003-3.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular / methods
  • DNA, Complementary
  • Electrophoresis, Polyacrylamide Gel / methods
  • Escherichia coli
  • GTP Phosphohydrolases / biosynthesis
  • GTP Phosphohydrolases / isolation & purification*
  • GTP-Binding Proteins / biosynthesis
  • GTP-Binding Proteins / isolation & purification*
  • Glutathione Transferase / biosynthesis
  • Guanosine Diphosphate / metabolism
  • Guanosine Triphosphate / metabolism
  • Mammals
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Point Mutation
  • Polymerase Chain Reaction / methods
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification*
  • Restriction Mapping
  • Schistosoma japonicum / enzymology
  • rac GTP-Binding Proteins

Substances

  • DNA, Complementary
  • Recombinant Fusion Proteins
  • Recombinant Proteins
  • Guanosine Diphosphate
  • Guanosine Triphosphate
  • Glutathione Transferase
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • rac GTP-Binding Proteins