[Purine arabinonucleoside-5'-triphosphates with substituents in the 2'-position as DNA polymerase substrates]

Mol Biol (Mosk). Jul-Aug 1995;29(4):918-29.
[Article in Russian]

Abstract

Analogues of araNTPs carrying an azido or aminogroup instead of the 2' hydroxyl exhibited substrate properties towards several mammalian and viral DNA polymerases. At the same time, introduction of a bulky hydrophobic DNP group into the 2' position inactivated the compounds as substrates. HSV-1 and CMV DNA polymerases were an interesting exception: they effectively incorporated the modified nucleotide residues with DNP group into the 3'-termini of the DNA chain. This is a reliable way to distinguish these enzymes from cellular DNA polymerases.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Arabinonucleotides / chemistry
  • Arabinonucleotides / metabolism*
  • Base Sequence
  • Chromatography, High Pressure Liquid
  • Cytomegalovirus / enzymology
  • DNA Primers
  • DNA-Directed DNA Polymerase / metabolism*
  • Herpesvirus 1, Human / enzymology
  • Molecular Sequence Data
  • Spectrophotometry, Ultraviolet
  • Substrate Specificity

Substances

  • Arabinonucleotides
  • DNA Primers
  • DNA-Directed DNA Polymerase