Development of specific DNA probes and their usage in the detection of Plasmodium vivax infection in blood

Mol Cell Probes. 1995 Aug;9(4):239-46. doi: 10.1016/s0890-8508(95)90103-5.


The application of nucleic acid probes, in the detection of pathogenic micro-organisms, has become an integral part of diagnostic technologies. In this study, Plasmodium vivax-specific DNA probes have been identified by carrying out genomic subtractive hybridization. In this approach, the recombinant clones from a P. vivax genomic library are screened with radiolabelled human and P. falciparum DNA. The colonies which react with labelled P. falciparum and human DNA are eliminated and those which do not produce any autoradiographic signal have been subjected to further screening procedures. Three P. vivax specific DNA probes have been obtained by these repeated screenings. Further analyses indicate that these probes are specific and sensitive enough to detect P. vivax infection in clinical blood samples when used in a non-radioactive DNA hybridization assay.

MeSH terms

  • Animals
  • Base Sequence
  • DNA Probes*
  • DNA, Protozoan / analysis*
  • DNA, Recombinant / analysis
  • Humans
  • Malaria, Vivax / blood
  • Malaria, Vivax / genetics
  • Malaria, Vivax / parasitology*
  • Molecular Sequence Data
  • Plasmodium vivax / isolation & purification*
  • Sensitivity and Specificity
  • Sequence Analysis


  • DNA Probes
  • DNA, Protozoan
  • DNA, Recombinant

Associated data

  • GENBANK/S79775
  • GENBANK/S79776
  • GENBANK/S79777