Effect of intake of exogenous vitamins C, E and beta-carotene on the antioxidative status in kidneys of rats with streptozotocin-induced diabetes

Nahrung. 1995;39(4):257-61. doi: 10.1002/food.19950390402.

Abstract

We studied the effect of supplementation with vitamins C, E and beta-carotene (PARABION, produced by Syndipharma) on antioxidative status in kidneys of male Wistar rats with diabetes induced by intravenous application of streptozotocin (45 mg.kg-1 of body weight). The animals received subtherapeutic doses of Insulin Interdep (6 U.kg-1 of body weight). A significant decrease of malondialdehyde (MDA), reduced (GSH) and oxidized (GSSG) glutathione and reduction of the activities of Se-glutathione peroxidase (Se-GSH-PX, EC. 1.11.1.9.) and glutathione S-transferase (GST, EC. 2.5.1.18.) were observed in kidneys of diabetic rats treated with these vitamins. On the contrary, the activity of CuZn-superoxide dismutase (CuZn-SOD, EC. 1.15.1.1) and the level of vitamin C (vit. C) increased significantly. No changes were observed for vitamin E (vit. E), beta-carotene and catalase (CAT, EC. 1.11.1.6). Supplementation with vitamins C, E and beta-carotene resulted in an improvement of antioxidative status of kidneys of rats with streptozotocin-induced diabetes.

MeSH terms

  • Animals
  • Antioxidants / metabolism*
  • Antioxidants / pharmacology*
  • Ascorbic Acid / pharmacology*
  • Body Weight / drug effects
  • Carotenoids / pharmacology*
  • Diabetes Mellitus, Experimental / metabolism*
  • Glutathione / metabolism
  • Kidney / drug effects
  • Kidney / metabolism*
  • Male
  • Malondialdehyde / metabolism
  • Organ Size / drug effects
  • Rats
  • Rats, Wistar
  • Vitamin E / pharmacology*
  • beta Carotene

Substances

  • Antioxidants
  • beta Carotene
  • Vitamin E
  • Carotenoids
  • Malondialdehyde
  • Glutathione
  • Ascorbic Acid