Mechanosensory signalling in C. elegans mediated by the GLR-1 glutamate receptor

Nature. 1995 Nov 2;378(6552):78-81. doi: 10.1038/378078a0.


NEURONAL signalling across synapses involves activation of many neurotransmitter receptors on postsynaptic cells. glr-1 encodes a potential glutamate receptor in the nematode Caenorhabditis elegans which is most similar to vertebrae AMPA-type ionotropic glutamate receptors. glr-1 is expressed in motor neurons and interneurons, including interneurons implicated in the control of locomotion. Here we investigate the contribution of glr-1 to the normal signalling of these neurons, by generating a deletion mutation in glr-1. We find that mutant worms are deficient in their ability to withdraw backwards when mechanically stimulated, but they withdraw normally in response to chemical repellents. The ASH sensory neurons mediate withdrawal responses both to mechanical stimuli and to repellents, and ASH makes chemical synapses with glr-1-expressing interneurons. Our results suggest that postsynaptic interneurons use different neurotransmitter receptors to process two sensory stimuli detected by one sensory neuron.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins*
  • DNA Primers
  • Interneurons / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Motor Neurons / metabolism
  • Mutagenesis, Insertional
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Neurons / metabolism*
  • Polymerase Chain Reaction
  • Receptors, AMPA
  • Receptors, Glutamate / genetics
  • Receptors, Glutamate / metabolism*
  • Sequence Deletion
  • Signal Transduction*
  • Synapses / physiology


  • Caenorhabditis elegans Proteins
  • DNA Primers
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Receptors, AMPA
  • Receptors, Glutamate
  • glr-1 protein, C elegans

Associated data

  • GENBANK/D13213
  • GENBANK/M36418
  • GENBANK/M36419
  • GENBANK/M36420
  • GENBANK/M36421
  • GENBANK/M73271
  • GENBANK/M91561
  • GENBANK/M91562
  • GENBANK/M91563
  • GENBANK/M97192
  • GENBANK/U34661
  • GENBANK/X59996
  • GENBANK/X60086
  • GENBANK/X63255
  • GENBANK/X71790
  • GENBANK/Z11581
  • GENBANK/Z11713
  • GENBANK/Z11715
  • GENBANK/Z11716
  • GENBANK/Z17238
  • GENBANK/Z17239