Down-regulation of blood-brain glucose transport in the hyperglycemic nonobese diabetic mouse

Neurochem Res. 1995 Jul;20(7):869-73. doi: 10.1007/BF00969700.

Abstract

The intracarotid injection method has been utilized to examine blood-brain barrier (BBB) glucose transport in hyperglycemic (4-6 days) mice. In anesthetized mice, Brain Uptake Indices were measured over a range of glucose concentrations from 0.010-50 mmol/l; glucose uptake was found to be saturable and kinetically characterized. The maximal velocity (Vmax) for glucose transport was 989 +/- 214 nmol.min-1.g-1. and the half-saturation constant estimated to be 5.80 +/- 1.38 mmol/l. The unsaturated Permeability Surface area product (PS) is = 171 + 8 microliters.min.-1.g-1. A rabbit polyclonal antiserum to a synthetic peptide encoding the 13 C-terminal amino acids of the human erythrocyte glucose transporter immunocytochemically confirmed the presence of the GLUT1 isoform in non-obese diabetic (NOD) mouse brain capillary endothelia. These studies indicate that a down-regulation of BBB glucose transport occurs in these spontaneously hyperglycemic mice; both BBB glucose permeability (as indicated by PS product) and transporter maximal velocity are reduced (in comparison to normoglycemic CD-1 mice), but the half-saturation constant remains unchanged.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blood Glucose / metabolism
  • Blood-Brain Barrier*
  • Capillaries
  • Cerebrovascular Circulation
  • Diabetes Mellitus, Type 1 / metabolism*
  • Endothelium / metabolism*
  • Erythrocytes / metabolism
  • Female
  • Glucose / metabolism*
  • Glucose Transporter Type 1
  • Humans
  • Hyperglycemia / metabolism*
  • Immune Sera
  • Kinetics
  • Mice
  • Mice, Inbred NOD
  • Mice, Inbred Strains
  • Monosaccharide Transport Proteins / analysis
  • Monosaccharide Transport Proteins / immunology
  • Monosaccharide Transport Proteins / metabolism*
  • Rabbits / immunology

Substances

  • Blood Glucose
  • Glucose Transporter Type 1
  • Immune Sera
  • Monosaccharide Transport Proteins
  • SLC2A1 protein, human
  • Slc2a1 protein, mouse
  • Glucose