Immunization with the paraneoplastic encephalomyelitis antigen HuD does not cause neurologic disease in mice

Neurology. 1995 Oct;45(10):1873-8. doi: 10.1212/wnl.45.10.1873.


Paraneoplastic encephalomyelitis/paraneoplastic sensory neuronopathy (PEM/PSN) associated with small cell lung cancer is characterized by high serum and CSF titers of anti-neuronal (anti-Hu) antibodies and by intrathecal synthesis of anti-Hu IgG. A pathologic role for the anti-Hu antibodies in PEM/PSN is further suggested by reported intraneuronal accumulation of the antibodies in the nervous system of PEM/PSN patients at autopsy. We immunized SJL/J mice, Lewis rats, and Hartley guinea pigs with purified recombinant HuD fusion protein. In spite of high-titer anti-HuD antibodies, neurologic and pathologic examination of the animals was normal. Apparent uptake of purified IgG by neurons in the brain proved to be artifactual.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies / administration & dosage
  • Antibody Formation
  • Base Sequence
  • Brain / pathology
  • ELAV Proteins
  • ELAV-Like Protein 4
  • Encephalomyelitis / immunology*
  • Encephalomyelitis / pathology
  • Guinea Pigs
  • Humans
  • Immunization
  • Immunohistochemistry
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins*
  • Paraneoplastic Syndromes / immunology*
  • Paraneoplastic Syndromes / pathology
  • RNA-Binding Proteins / administration & dosage
  • RNA-Binding Proteins / immunology*
  • Rats


  • Antibodies
  • ELAV Proteins
  • ELAV-Like Protein 4
  • ELAVL4 protein, human
  • Elavl4 protein, mouse
  • Nerve Tissue Proteins
  • RNA-Binding Proteins