Bik, a novel death-inducing protein shares a distinct sequence motif with Bcl-2 family proteins and interacts with viral and cellular survival-promoting proteins

Oncogene. 1995 Nov 2;11(9):1921-8.

Abstract

The survival-promoting activity of the Bcl-2 family of proteins appears to be modulated by interactions between various cellular proteins. We have identified a novel cellular protein, Bik, that interacts with the cellular survival-promoting proteins, Bcl-2 and Bcl-xL, as well as the viral survival-promoting proteins, Epstein Barr virus-BHRF1 and adenovirus E1B-19 kDa. In transient transfection assays, Bik promotes cell death in a manner similar to the death-promoting members of the Bcl-2 family, Bax and Bak. This death-promoting activity of Bik can be suppressed by coexpression of Bcl-2, Bcl-XL, EBV-BHRF1 and E1B-19 kDa proteins suggesting that Bik may be a common target for both cellular and viral anti-apoptotic proteins. While Bik does not show overt homology to the BH1 and BH2 conserved domains characteristic of the Bcl-2 family, it does share a 9 amino acid domain (BH3) with Bax and Bak which may be a critical determinant for the death-promoting activity of these proteins.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoviridae / metabolism
  • Adenovirus E1B Proteins / chemistry
  • Adenovirus E1B Proteins / metabolism
  • Amino Acid Sequence
  • Animals
  • Apoptosis Regulatory Proteins
  • B-Lymphocytes / metabolism
  • Base Sequence
  • Cell Death
  • Cell Line
  • Fibroblasts
  • Gene Library
  • Herpesvirus 4, Human / metabolism
  • Humans
  • Membrane Proteins*
  • Mitochondrial Proteins
  • Molecular Sequence Data
  • Protein Kinase C / metabolism
  • Proteins / chemistry*
  • Proteins / metabolism
  • Proto-Oncogene Proteins / chemistry*
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-bcl-2
  • Rats
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Substrate Specificity
  • Transfection
  • Viral Proteins / chemistry*
  • Viral Proteins / metabolism
  • bcl-X Protein

Substances

  • Adenovirus E1B Proteins
  • Apoptosis Regulatory Proteins
  • BCL2L1 protein, human
  • BHRF1 protein, Human herpesvirus 4
  • BIK protein, human
  • Bcl2l1 protein, rat
  • Membrane Proteins
  • Mitochondrial Proteins
  • Proteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Recombinant Proteins
  • Viral Proteins
  • bcl-X Protein
  • Protein Kinase C

Associated data

  • GENBANK/U34584