The purification and protective capacity of Bordetella pertussis outer membrane proteins

Vaccine. 1995;13(8):747-52. doi: 10.1016/0264-410x(94)00040-t.

Abstract

The whole cell vaccine (WCV) of Bordetella pertussis is protective in the intracerebral (i.c.) mouse protection assay. We found a correlation between the i.c. mouse protection assay potency and the presence of the virulence-associated outer membrane proteins (OMPs) in outer membrane complexes (OMC). The virulence-associated 92, 32 and 30 kDa OMPs were purified and the N-terminal amino acid sequences were determined. The N-terminal amino acid sequences of the 30 and 32 kDa OMPs show homology with the C-terminal fragment of the P.93 precursor of the 69 kDa OMP (pertactin). The purified 32 kDa OMP was protective in the i.c. test when presented as mixed protein-detergent micelles. The 92 kDa OMP became a protective antigen when nonprotective levels of pertussis toxin were added. We found a correlation between the i.c. mouse protection value and the 92 kDal38 kDa (porin) ratio in OMC preparations.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacterial Outer Membrane Proteins / administration & dosage
  • Bacterial Outer Membrane Proteins / immunology
  • Bacterial Outer Membrane Proteins / isolation & purification*
  • Bacterial Vaccines / chemistry
  • Bacterial Vaccines / immunology*
  • Bacterial Vaccines / therapeutic use
  • Bordetella Infections / microbiology
  • Bordetella Infections / prevention & control*
  • Bordetella pertussis / immunology*
  • Bordetella pertussis / pathogenicity
  • Drug Compounding
  • Female
  • Injections, Intraventricular
  • Male
  • Mice
  • Micelles
  • Molecular Sequence Data

Substances

  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines
  • Micelles