Involvement of PIM-1 in DNA fragmentation in mouse NS-1-derived cells

Biochem Biophys Res Commun. 1995 Oct 13;215(2):538-46. doi: 10.1006/bbrc.1995.2498.


In several cell lines derived from mouse NS-1 myeloma cells, internucleosomal fragmentation of chromosomal DNA, a hallmark of apoptosis, was continuously observed. Approximately 15-20% of the cells died when isolated in a 96-well plate, and the surviving cells contained fragmented DNA ('ladder'). Among a variety of genes so far reported to be related to apoptosis, only Pim-1 was expressed at an elevated level in the NS-1 hybridomas as compared in a control myeloma cell line without 'ladder'. Transfection of a Pim-1 expression vector to a 'ladder'-non-producing myeloma line yielded similar internucleosomal DNA fragmentation. The results hence suggested that Pim-1 activates endonucleases responsible for DNA fragmentation during apoptosis and/or repress DNA repair systems to restore fragmented DNA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Base Sequence
  • Cell Line
  • Clone Cells
  • DNA Damage
  • DNA Primers
  • DNA Probes
  • DNA, Neoplasm / isolation & purification
  • DNA, Neoplasm / metabolism*
  • Flow Cytometry
  • Gene Expression
  • Hybridomas
  • Mice
  • Molecular Sequence Data
  • Multiple Myeloma
  • Plasmids
  • Polymerase Chain Reaction
  • Protein Serine-Threonine Kinases / metabolism*
  • Proto-Oncogene Proteins / biosynthesis
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-pim-1
  • Tumor Cells, Cultured


  • DNA Primers
  • DNA Probes
  • DNA, Neoplasm
  • Proto-Oncogene Proteins
  • Pim1 protein, mouse
  • Protein Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-pim-1