Expression of the chemokine receptor BLR2/EBI1 is specifically transactivated by Epstein-Barr virus nuclear antigen 2

Biochem Biophys Res Commun. 1995 Oct 13;215(2):737-43. doi: 10.1006/bbrc.1995.2525.


In our attempt to identify chemokine receptors that are related to Burkitt's lymphoma receptor 1 (BLR1) and are expressed in activated lymphocytes we used RT-PCR resulting in the isolation of a cDNA encoding a seven transmembrane receptor termed BLR2. The protein shows significant sequence similarities to the family of G-protein coupled chemokine receptors and turned out to be identical to the recently described receptor EBI1. Northern blot analysis revealed that BLR2 mRNA could be highly stimulated in mitogen- and anti-CD3-treated peripheral blood lymphocytes. BLR2-specific mRNA could be detected in all Epstein-Barr virus positive B cell lines. We show that transcription of the BLR2 gene could be specifically induced in Epstein-Barr virus negative BL 41 cells via estrogen-mediated activation of Epstein-Barr virus nuclear antigen 2, a key regulator of viral and cellular genes in immortalized B cells. Our data suggest an involvement of BLR2 in the regulation of migration in activated lymphocytes and in viral pathogenesis.

MeSH terms

  • Antigens, Viral / metabolism*
  • B-Lymphocytes / immunology
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA Primers
  • DNA, Complementary
  • DNA-Binding Proteins / metabolism*
  • Epstein-Barr Virus Nuclear Antigens
  • Gene Expression
  • Herpesvirus 4, Human / physiology*
  • Humans
  • Lymphocyte Activation
  • Lymphocytes / immunology*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Receptors, CCR7
  • Receptors, Cell Surface / biosynthesis*
  • Receptors, Chemokine*
  • Trans-Activators / metabolism*
  • Transcription, Genetic
  • Transcriptional Activation*


  • Antigens, Viral
  • CCR7 protein, human
  • DNA Primers
  • DNA, Complementary
  • DNA-Binding Proteins
  • Epstein-Barr Virus Nuclear Antigens
  • RNA, Messenger
  • Receptors, CCR7
  • Receptors, Cell Surface
  • Receptors, Chemokine
  • Trans-Activators

Associated data

  • GENBANK/X84702