Midkine is a heat and acid stable polypeptide capable of enhancing plasminogen activator activity and neurite outgrowth extension

Biochem Biophys Res Commun. 1995 Nov 13;216(2):574-81. doi: 10.1006/bbrc.1995.2661.


We studied about the physicochemical stability of a novel heparin-binding growth/differentiation factor, midkine (MK). It was found that synthetic human MK was heat and acid stable. Neither incubation at 80 degrees C for 90 sec nor treatment at low pH affected the elution profile of MK molecule on the high performance liquid chromatography. This physicochemical stability was maintained in the biological activities of MK to enhance plasminogen activator (PA) activity in bovine endothelial cells as well as to promote neurite outgrowth of rat brain cells. A similar stability was observed both with recombinant murine MK and with its homologous protein, recombinant human pleiotrophin. Comparison of physicochemical stability with other several growth factors suggested that MK/pleiotrophin was a unique family of heat and acid stable polypeptides capable of enhancing PA activity and neurite outgrowth.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta
  • Brain / physiology
  • Carrier Proteins / chemistry*
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / pharmacology*
  • Cattle
  • Chromatography, High Pressure Liquid
  • Cytokines / chemistry
  • Cytokines / pharmacology*
  • Drug Stability
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Humans
  • Hydrogen-Ion Concentration
  • Kinetics
  • Midkine
  • Mitogens / pharmacology
  • Neurites / drug effects
  • Neurites / physiology
  • Neurons / drug effects
  • Neurons / physiology*
  • Neurons / ultrastructure
  • Plasminogen Activators / metabolism*
  • Rats
  • Recombinant Proteins / pharmacology
  • Thermodynamics


  • Carrier Proteins
  • Cytokines
  • Mitogens
  • Recombinant Proteins
  • pleiotrophin
  • Midkine
  • Plasminogen Activators