NMDA receptor subunits epsilon 1 (NR2A) and epsilon 2 (NR2B) are substrates for Fyn in the postsynaptic density fraction isolated from the rat brain

Biochem Biophys Res Commun. 1995 Nov 13;216(2):582-8. doi: 10.1006/bbrc.1995.2662.


Fyn, protein tyrosine kinase, and its substrates were highly concentrated in the postsynaptic density (PSD) fraction prepared from the rat forebrain. There were a number of Fyn substrates unique to the PSD fraction. One of the major substrates in the PSD fraction was found to be a concanavalin A-binding glycoprotein, PSD-gp180, which is the N-methyl-D-aspartate (NMDA) receptor subunit epsilon 2 (NR2B). Western blotting and immunoprecipitation supported the phosphorylation of epsilon 2 by Fyn. NMDA receptor subunit epsilon 1 (NR2A) was also a substrate for Fyn. These results suggest that Fyn is involved in the modulation of synaptic efficacy through the phosphorylation of synapse-specific substrates such as the NMDA receptor/channel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Fractionation
  • Concanavalin A
  • Macromolecular Substances
  • Phosphorylation
  • Phosphotyrosine / analysis
  • Prosencephalon / metabolism*
  • Protein-Tyrosine Kinases / metabolism*
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-fyn
  • Rats
  • Rats, Wistar
  • Receptors, N-Methyl-D-Aspartate / chemistry
  • Receptors, N-Methyl-D-Aspartate / isolation & purification
  • Receptors, N-Methyl-D-Aspartate / metabolism*
  • Subcellular Fractions / enzymology
  • Substrate Specificity
  • Synapses / metabolism*


  • Macromolecular Substances
  • Proto-Oncogene Proteins
  • Receptors, N-Methyl-D-Aspartate
  • Concanavalin A
  • Phosphotyrosine
  • Protein-Tyrosine Kinases
  • Fyn protein, rat
  • Proto-Oncogene Proteins c-fyn