Association of HLA-B39 with HLA-B27-negative ankylosing spondylitis and pauciarticular juvenile rheumatoid arthritis in Japanese patients. Evidence for a role of the peptide-anchoring B pocket

Arthritis Rheum. 1995 Nov;38(11):1672-7. doi: 10.1002/art.1780381120.


Objective: To investigate the characteristics of HLA-B27 that render susceptibility to seronegative spondylarthropathies.

Methods: Serologic HLA class I typing of Japanese patients with ankylosing spondylitis (AS), juvenile rheumatoid arthritis (JRA), and healthy controls, was performed. HLA-B39 subtypes were determined by polymerase chain reaction-sequence-specific oligohybridization.

Results: HLA-B27 was present in 40 of 48 patients with AS (83%), and in only 1 of 210 healthy controls (0.5%). Three of 8 patients (37.5%) who were negative for HLA-B27 were positive for HLA-B39, which was significantly higher compared with the HLA-B27-negative controls (6.2%; P = 0.01). Significant association with HLA-B39 was also noted in the JRA patients (16.7%; P < 0.01), especially in those patients with pauciarticular-onset disease (33.3%; P < 0.01). Ten of 13 HLA-B39-positive patients had subtype B*3901 and 3 had B*3902.

Conclusion: Because HLA-B27 and HLA-B39 share Glu at position 45 and Cys at position 67, both of which constitute components of the peptide-anchoring B pocket, and because they possess similar peptide-ligand motifs, our results may support either the role of the peptides presented by class I antigens or the importance of Cys at position 67, in the development of spondylarthropathies and pauciarticular-onset JRA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis, Juvenile / blood
  • Arthritis, Juvenile / epidemiology*
  • Arthritis, Juvenile / immunology*
  • Base Sequence
  • HLA-B Antigens / analysis*
  • HLA-B27 Antigen / analysis*
  • HLA-B39 Antigen
  • Histocompatibility Antigens Class I / analysis
  • Histocompatibility Testing
  • Humans
  • Japan / epidemiology
  • Molecular Sequence Data
  • Spondylitis, Ankylosing / blood
  • Spondylitis, Ankylosing / epidemiology*
  • Spondylitis, Ankylosing / immunology*


  • HLA-B Antigens
  • HLA-B27 Antigen
  • HLA-B39 Antigen
  • Histocompatibility Antigens Class I