Calcium signals in olfactory neurons

Biochim Biophys Acta. 1995 Nov 9;1269(2):129-38. doi: 10.1016/0167-4889(95)00105-2.

Abstract

Laser scanning confocal microscopy in combination with the fluorescent calcium indicators Fluo-3 and Fura-Red was employed to estimate the intracellular concentration of free calcium ions in individual olfactory receptor neurons and to monitor temporal and spatial changes in the Ca(2+)-level upon stimulation. The chemosensory cells responded to odorants with a significant increase in the calcium concentration, preferentially in the dendritic knob. Applying various stimulation paradigma, it was found that in a population of isolated cells, subsets of receptor neurons display distinct patterns of responsiveness.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds
  • Animals
  • Benzofurans
  • Biological Transport
  • Calcium / metabolism*
  • Dose-Response Relationship, Drug
  • Fluorescent Dyes
  • Imidazoles
  • In Vitro Techniques
  • Lasers
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Odorants
  • Olfactory Receptor Neurons / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Signal Transduction*
  • Xanthenes

Substances

  • Aniline Compounds
  • Benzofurans
  • Fluorescent Dyes
  • Imidazoles
  • Xanthenes
  • fura red
  • Fluo-3
  • Calcium