Administration of interleukin-5 or -10 activates peritoneal B-1 cells and induces autoimmune hemolytic anemia in anti-erythrocyte autoantibody-transgenic mice

Eur J Immunol. 1995 Nov;25(11):3047-52. doi: 10.1002/eji.1830251110.


Activation mechanisms of B-1 (Ly-1 B) cells have been suggested to be different from those of conventional B cells. To assess the role of various interleukins (IL) in the activation of B-1 cells, we injected IL-4, IL-5 or IL-10 into nonanemic anti-red blood cells (RBC) autoantibody-transgenic mice, in which conventional B cells are clonally deleted but peritoneal B-1 cells persist without secreting Ig. Intraperitoneal or intramuscular injection of IL-5 or IL-10, but not IL-4, increased the number of antibody-producing peritoneal B-1 cells by four- to five-fold, resulting in increased anti-RBC serum autoantibody and induction of hemolytic anemia. These results suggest that IL-5 or IL-10 may play an important role in the terminal differentiation of B-1 cells into antibody-producing cells in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anemia, Hemolytic, Autoimmune / chemically induced*
  • Animals
  • Autoantibodies / biosynthesis
  • Autoantibodies / immunology
  • B-Lymphocytes / drug effects*
  • Cell Differentiation / drug effects
  • Erythrocytes / immunology
  • Interleukin-10 / administration & dosage
  • Interleukin-10 / pharmacology*
  • Interleukin-4 / administration & dosage
  • Interleukin-4 / pharmacology
  • Interleukin-5 / administration & dosage
  • Interleukin-5 / pharmacology*
  • Lymphocyte Activation / drug effects
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Peritoneal Cavity / cytology


  • Autoantibodies
  • Interleukin-5
  • Interleukin-10
  • Interleukin-4