Der p I, a major allergen of the house dust mite, proteolytically cleaves the low-affinity receptor for human IgE (CD23)

Eur J Immunol. 1995 Nov;25(11):3191-4. doi: 10.1002/eji.1830251131.

Abstract

The nature of the proteases that cleave CD23 in vivo is of considerable interest, but remains unknown. Here, we demonstrate that Der p I, a major allergen of the house dust mite Dermatophagoides pteronyssinus, cleaves CD23 from the surface of cultured human B cells (RPMI 8866 B cell line). The cleavage of the receptor from the B cell surface was associated with a parallel increase in soluble CD23 (sCD23) in the culture supernatant. Furthermore, the proteolytic effect of Der p I was specific for CD23, since none of the other B cell markers tested (CD20, HLA-DR, CD71 and CD49d) were affected. Labeled antibody experiments and protease inhibition assays clearly demonstrate that Der p I is a cysteine protease that directly cleaves a 25-kDa fragment of CD23. These data suggest that the cysteine protease Der p I, in addition to being highly immunogenic, may up-regulate IgE synthesis by virtue of its ability to cleave CD23.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / pharmacology*
  • Amino Acid Sequence
  • Animals
  • Antigens, Dermatophagoides
  • B-Lymphocytes / drug effects
  • B-Lymphocytes / immunology*
  • Cells, Cultured
  • Cysteine Endopeptidases / pharmacology*
  • Glycoproteins / pharmacology*
  • Humans
  • Mites
  • Molecular Sequence Data
  • Receptors, IgE / analysis
  • Receptors, IgE / metabolism*

Substances

  • Allergens
  • Antigens, Dermatophagoides
  • Glycoproteins
  • Receptors, IgE
  • Cysteine Endopeptidases