Green fluorescent protein production in the cellular slime molds Polysphondylium pallidum and Dictyostelium discoideum

Gene. 1995 Nov 7;165(1):127-30. doi: 10.1016/0378-1119(95)00430-e.

Abstract

The green fluorescent protein-encoding gene from Aequorea victoria has been cloned into several different transforming vectors and expressed in the cellular slime molds, Polysphondylium pallidum and Dictyostelium discoideum. We find that the protein is stable and non-toxic in both species, can be easily visualized in living and fixed specimens, and can be used to purify rare cells by fluorescence-activated cell sorting (FACS).

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Dictyostelium / genetics*
  • Dictyostelium / metabolism
  • Flow Cytometry
  • Gene Transfer Techniques
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Luminescent Proteins / genetics*
  • Luminescent Proteins / isolation & purification
  • Luminescent Proteins / metabolism
  • Molecular Sequence Data

Substances

  • Luminescent Proteins
  • Green Fluorescent Proteins