New plasmids carrying antibiotic-resistance cassettes

Gene. 1995 Nov 7;165(1):141-2. doi: 10.1016/0378-1119(95)00529-f.

Abstract

A series of new plasmid vectors is described that carry gene cassettes imparting resistance to the antibiotics chloramphenicol (CmR), kanamycin (KmR), tetracycline (TcR) and spectinomycin/streptomycin (Sp/SmR). The gene cassettes are symmetrically flanked by several restriction sites. In addition, several restriction sites that are normally found internal to the gene cassettes have been eliminated, thereby expanding the number of restriction enzymes available to excise an intact antibiotic-resistance gene. The gene cassettes are carried by high-copy-number plasmids that confer ampicillin resistance (ApR).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Transposable Elements / genetics*
  • Drug Resistance, Microbial / genetics*
  • Escherichia coli / genetics
  • Plasmids / genetics*
  • Plasmids / isolation & purification

Substances

  • DNA Transposable Elements