Analysis of recombinant soluble mouse D-factor/LIF receptor

J Biochem. 1995 Jun;117(6):1228-31. doi: 10.1093/oxfordjournals.jbchem.a124848.

Abstract

The recombinant soluble mouse D-factor/LIF receptor (sD-factor-R) was expressed in COS-7 cells. Scatchard analysis of the bindings of mouse 125I-D-factor and human 125I-D-factor to the sD-factor-R indicated dissociation constants (Kd) of 12 and 0.267 nM, respectively, which were comparable to those of the binding protein in mouse serum. The apparent molecular masses of the sD-factor-R and human D-factor observed by gel filtration chromatography were 150 and 50 kDa, respectively. The size of the sD-factor-R.human D-factor complex was approximately 200 kDa, indicating that D-factor forms a 1:1 complex with the sD-factor-R. The sD-factor-R inhibited the induction of differentiation of mouse myeloid leukemic M1 cells by mouse D-factor by blocking the binding of this factor to the cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Chromatography, Gel
  • Growth Inhibitors / metabolism*
  • Growth Inhibitors / pharmacology
  • Humans
  • Interleukin-6*
  • Leukemia Inhibitory Factor
  • Leukemia Inhibitory Factor Receptor alpha Subunit
  • Lymphokines / metabolism*
  • Lymphokines / pharmacology
  • Mice
  • Phagocytosis
  • Receptors, Cytokine / chemistry
  • Receptors, Cytokine / genetics
  • Receptors, Cytokine / metabolism*
  • Receptors, OSM-LIF
  • Recombinant Proteins / metabolism
  • Solubility
  • Tumor Cells, Cultured

Substances

  • Growth Inhibitors
  • Interleukin-6
  • LIF protein, human
  • LIFR protein, human
  • Leukemia Inhibitory Factor
  • Leukemia Inhibitory Factor Receptor alpha Subunit
  • Lif protein, mouse
  • Lifr protein, mouse
  • Lymphokines
  • Receptors, Cytokine
  • Receptors, OSM-LIF
  • Recombinant Proteins