Binding between the neural cell adhesion molecules axonin-1 and Nr-CAM/Bravo is involved in neuron-glia interaction

J Cell Biol. 1995 Nov;131(4):1067-81. doi: 10.1083/jcb.131.4.1067.


Neural cell adhesion molecules of the immunoglobulin superfamily mediate cellular interactions via homophilic binding to identical molecules and heterophilic binding to other family members or structurally unrelated cell-surface glycoproteins. Here we report on an interaction between axonin-1 and Nr-CAM/Bravo. In search for novel ligands of axonin-1, fluorescent polystyrene microspheres conjugated with axonin-1 were found to bind to peripheral glial cells from dorsal root ganglia. By antibody blockage experiments an axonin-1 receptor on the glial cells was identified as Nr-CAM. The specificity of the interaction was confirmed with binding studies using purified axonin-1 and Nr-CAM. In cultures of dissociated dorsal root ganglia antibodies against axonin-1 and Nr-CAM perturbed the formation of contacts between neurites and peripheral glial cells. Together, these results implicate a binding between axonin-1 of the neuritic and Nr-CAM of the glial cell membrane in the early phase of axon ensheathment in the peripheral nervous system.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibody Specificity
  • Axons / physiology
  • Base Sequence
  • Cell Adhesion / physiology
  • Cell Adhesion Molecules*
  • Cell Adhesion Molecules, Neuronal / immunology
  • Cell Adhesion Molecules, Neuronal / metabolism*
  • Chick Embryo
  • Contactin 2
  • DNA Primers / chemistry
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / embryology
  • Integrins / physiology
  • Microspheres
  • Molecular Sequence Data
  • Neurites / ultrastructure
  • Neuroglia / cytology*
  • Neuroglia / ultrastructure
  • Neurons / cytology*
  • Neurons / ultrastructure
  • Protein Binding / physiology


  • CNTN2 protein, human
  • Cell Adhesion Molecules
  • Cell Adhesion Molecules, Neuronal
  • Contactin 2
  • DNA Primers
  • Integrins
  • NRCAM protein, human