Abnormal Lung Development and Cleft Palate in Mice Lacking TGF-beta 3 Indicates Defects of Epithelial-Mesenchymal Interaction

Nat Genet. 1995 Dec;11(4):415-21. doi: 10.1038/ng1295-415.

Abstract

A broad spectrum of biological activities has been proposed for transforming growth factor-beta 3 (TGF-beta 3). To study TGF-beta 3 function in development, TGF-beta 3 null mutant mice were generated by gene-targeting. Within 20 hours of birth, homozygous TGF-beta 3-/- mice die with unique and consistent phenotypic features including delayed pulmonary development and defective palatogenesis. Unlike other null mutants with cleft palate, TGF-beta 3-/- mice lack other concomitant craniofacial abnormalities. This study demonstrates an essential function for TGF-beta 3 in the normal morphogenesis of palate and lung, and directly implicates this cytokine in mechanisms of epithelial-mesenchymal interaction.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Base Sequence
  • Cleft Palate / embryology
  • Cleft Palate / genetics*
  • Epithelium / physiology
  • Lung / abnormalities*
  • Lung / chemistry
  • Lung / embryology
  • Mesoderm / physiology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Sequence Data
  • Morphogenesis
  • Palate / embryology*
  • Proteolipids / analysis
  • Pulmonary Surfactants / analysis
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / physiology*

Substances

  • Proteolipids
  • Pulmonary Surfactants
  • Transforming Growth Factor beta