Transfected type II interleukin-1 receptor impairs responsiveness of human keratinocytes to interleukin-1

Am J Pathol. 1995 Dec;147(6):1852-61.


Of the two known types of specific receptors for interleukin (IL)-1, the function of the type II IL-1 receptor (IL-1RII) is still elusive. IL-1RII is allegedly devoid of signaling capacity and is therefore thought to act by trapping and inhibiting IL-1. To directly assess the functional role of IL-1RII, a human keratinocyte cell line has been stably transfected with a cDNA coding for IL-1RII, and its responsiveness to IL-1 has been compared with that of nontransfected cells. Parental cells express IL-1RI and are responsive to low doses of IL-1, whereas transfected cells overexpress IL-1RII, both in its membrane and soluble form, and show a dramatically impaired response to IL-1. Selective block of IL-1RII restores the ability of transfected keratinocytes to respond to IL-1, indicating that the overexpressed IL-1RII is in fact uniquely responsible for their refractoriness to IL-1. The main mechanism of unresponsiveness in transfected keratinocytes appears to be the capture and neutralization of IL-1 by the soluble form of IL-1RII.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Line
  • Humans
  • Interleukin-1 / metabolism
  • Interleukin-1 / pharmacology*
  • Keratinocytes / drug effects*
  • Keratinocytes / metabolism
  • Molecular Sequence Data
  • Receptors, Interleukin-1 / biosynthesis
  • Receptors, Interleukin-1 / genetics*
  • Receptors, Interleukin-1 / physiology*
  • Transfection / genetics*


  • Interleukin-1
  • Receptors, Interleukin-1