CD86 (B70/B7-2) on endothelial cells co-stimulates allogeneic CD4+ T cells

Int Immunol. 1995 Aug;7(8):1331-7. doi: 10.1093/intimm/7.8.1331.


In vascularized organ transplantation, vascular endothelial cells (EC) confronting recipient T cells are potentially significant APC initiating cellular immune responses that lead to rejection. In the present study, we studied the ability of human EC to stimulate allogeneic T cells and the co-stimulatory molecules involved in this response. On both human umbilical vein endothelial cells (HUVEC) and microvascular endothelial cells (MVEC), MHC class I, intercellular adhesion molecule (ICAM)-1 and CD86 were constitutively expressed as assessed by flow cytometry. After IFN-gamma treatment, MHC class II expression was induced, and MHC class I and ICAM-1 were up-regulated. In contrast, the expression of CD86 was unchanged and CD80 was undetectable even after IFN-gamma treatment. Highly purified CD4+ T cells proliferated in response to IFN-gamma-treated allogeneic HUVEC and MVEC, and this response was efficiently blocked by mAb to MHC class II, ICAM-1 and CD86. Furthermore, the addition of anti-CD86 mAb to the primary culture with allogeneic EC resulted in the induction of alloantigen-specific anergy. These results suggest that CD86 expressed on EC plays a critical role in initiating cellular immune responses to vascularized allografts and would be an important target for immune intervention.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / pharmacology
  • Antigens, CD / immunology
  • Antigens, CD / physiology*
  • B7-2 Antigen
  • CD4-Positive T-Lymphocytes / immunology*
  • Cell Adhesion Molecules / metabolism
  • Clonal Anergy / immunology
  • Endothelium, Vascular / chemistry
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / immunology*
  • Humans
  • Isoantigens / genetics*
  • Isoantigens / immunology
  • Lymphocyte Activation*
  • Major Histocompatibility Complex / immunology
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / physiology*
  • Skin / blood supply
  • Umbilical Veins


  • Antibodies, Monoclonal
  • Antigens, CD
  • B7-2 Antigen
  • CD86 protein, human
  • Cell Adhesion Molecules
  • Isoantigens
  • Membrane Glycoproteins