Cloning and primary structure of a new hemolysin gene from Bacillus cereus

Biochim Biophys Acta. 1995 Nov 7;1264(2):151-4. doi: 10.1016/0167-4781(95)00150-f.

Abstract

A new hemolysin gene from Bacillus cereus VKM-B164 was cloned in Escherichia coli and sequenced. Deduced protein consists of 219 amino acids and has a molecular mass of 24.4 kDa. It has been concluded that the hemolysin, named 'hemolysin III', is distinct from the B. cereus hemolysins reported previously: cereolysin, sphingomyelinase, cereolysin AB, hemolysin II, and 'cereolysin-like' hemolysin (Honda, T., Shiba, A., Seo, S., Yamamoto, J., Matsuyama, J. and Miwatani, T. (1991) FEMS Microbiol. Lett. 79, 205-210).

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Bacillus cereus / genetics*
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular / methods
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / metabolism
  • Escherichia coli
  • Genes, Bacterial*
  • Hemolysin Proteins / biosynthesis
  • Hemolysin Proteins / chemistry
  • Hemolysin Proteins / genetics*
  • Molecular Sequence Data
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Restriction Mapping

Substances

  • DNA, Bacterial
  • Hemolysin Proteins
  • Recombinant Proteins

Associated data

  • GENBANK/X84058