Expression of the T antigen on a T-lymphoid cell line, supT1

Glycoconj J. 1995 Jun;12(3):356-9. doi: 10.1007/BF00731338.

Abstract

We have measured glycosyltransferase activities of SupT1 cells, a T-lymphoid cell line shown to react with autoantibodies in the sera of many HIV patients. Since considerable alpha-N-acetylgalactosaminyl-transferase and beta 1, 3 galactosyltransferase activities were found in SupT1 cells, at least the O-glycan core 1 structure can probably be synthesized. FACS analysis using an anti-T monoclonal antibody showed expression of the T antigen (Gal beta 1-3 GalNAc). Glycoproteins with the T antigen were isolated by immunoprecipitation with the anti-T antibody from a SupT1 cell lysate labelled metabolically with 3H-glucosamine and then analysed by SDS-PAGE. It was revealed that the precipitate contained a glycoprotein with a molecular weight corresponding to that of leukosialin. O-glycans were prepared from the immunoprecipitate by alkaline-borohydride treatment and then fractionated on Bio-Gel P-2, GalNAcOH and Gal-GalNAcOH being identified inter alia. These results suggest that an anti-T antibody may be included in the autoantibodies found in HIV-1 infected individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / blood*
  • Antigens, Viral, Tumor / blood*
  • Autoantibodies / blood
  • Carbohydrate Sequence
  • Cell Line
  • HIV-1 / immunology*
  • Humans
  • Leukosialin
  • Molecular Sequence Data
  • Polysaccharides / blood
  • Sialoglycoproteins / immunology*
  • T-Lymphocytes / immunology*

Substances

  • Antigens, CD
  • Antigens, Viral, Tumor
  • Autoantibodies
  • Leukosialin
  • Polysaccharides
  • SPN protein, human
  • Sialoglycoproteins