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, 16 (6), 948-51

A Silver Stain Protocol for Proteins Yielding High Resolution and Transparent Background in Sodium Dodecyl Sulfate-Polyacrylamide Gels

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A Silver Stain Protocol for Proteins Yielding High Resolution and Transparent Background in Sodium Dodecyl Sulfate-Polyacrylamide Gels

M Swain et al. Electrophoresis.

Abstract

A simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/ acetic acid and prior to glutaraldehyde cross-linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.

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