Simulium damnosum s.l.: identification of inducible serine proteases following an Onchocerca infection by differential display reverse transcription PCR

Exp Parasitol. 1995 Nov;81(3):249-54. doi: 10.1006/expr.1995.1115.

Abstract

Vector-derived proteases are thought to be key to the regulation of filarial infections in Simulium damnosum s.I. To identify proteases of S. damnosum s.I. induced by infection with Onchocerca ochengi, a PCR-based differential display technique was used. By combining this method with homology-based serine protease primers transcripts can be detected from S. damnosum s.I. RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Primers / chemistry
  • DNA, Complementary / chemistry
  • Gene Expression Regulation, Enzymologic
  • Insect Vectors / enzymology*
  • Insect Vectors / genetics
  • Insect Vectors / parasitology
  • Molecular Sequence Data
  • Onchocerca / physiology*
  • Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Serine Endopeptidases / analysis*
  • Serine Endopeptidases / chemistry
  • Serine Endopeptidases / genetics
  • Simuliidae / enzymology*
  • Simuliidae / genetics
  • Simuliidae / parasitology
  • Transcription, Genetic

Substances

  • DNA Primers
  • DNA, Complementary
  • Serine Endopeptidases