Unusual long target duplication by insertion of intracisternal A-particle element in radiation-induced acute myeloid leukemia cells in mouse

FEBS Lett. 1995 Dec 4;376(3):146-50. doi: 10.1016/0014-5793(95)01262-2.

Abstract

Retrotransmission into the IL-3/GM-CSF gene locus by the retrotransposon intracisternal A-particle (IAP) had been observed in distinct tumor cell lines. We analyzed the locus in genomes from 7 different myeloid leukemia cell strains which were originally generated by whole-body X-irradiation of the inbred C3H/He mice at a dose of 3 Gy and maintained by in vivo passage. In one leukemia cell strain out of 7 such cases, RFLP of an allele of the interleukin-3 gene was found. Sequence analysis after cloning from the genomic library showed that a type I delta 2 IAP element was inserted in the region upstream of the IL-3 gene in the head-to-head orientation. This suggests that the locus in myeloid cells is sensitive for integration of IAP elements. Additionally, an unusual long target duplication of 82 bp, 14-fold larger than normal one, was found at the junction of the element. This suggests the possibility of a radiation-induced integration mechanism which is distinct from normal retrotransmission.

MeSH terms

  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / chemistry
  • DNA, Neoplasm / genetics*
  • Female
  • Gene Rearrangement
  • Genes, Intracisternal A-Particle / genetics*
  • Interleukin-3 / genetics
  • Leukemia, Experimental / genetics*
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Radiation-Induced / genetics*
  • Male
  • Mice
  • Mice, Inbred C3H
  • Molecular Sequence Data
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Retroelements / genetics*
  • Virus Integration*
  • X-Rays

Substances

  • DNA Primers
  • DNA, Neoplasm
  • Interleukin-3
  • Retroelements

Associated data

  • GENBANK/D63766
  • GENBANK/D63767