Efficient regulation of gene expression by adenovirus vector-mediated delivery of the CRE recombinase

Biochem Biophys Res Commun. 1995 Dec 14;217(2):393-401. doi: 10.1006/bbrc.1995.2789.


We have constructed an E1-defective adenovirus (Ad) vector designated AdCAG-Cre containing the Cre recombinase gene derived from bacteriophage P1 under control of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid (CAG) promoter. We examined the Cre-loxP-based recombination by this Ad vector in C2C12 cells bearing a reporter gene construct CAG-CAT-Z, which directs expression of the E. coli lacZ gene upon Cre-mediated excision of the CAT gene located between the CAG promoter and the lacZ gene. Nearly 100% of these cells were shown to start to produce beta-galactosidase after infection with the AdCAG-Cre vector at MOI 100. On the basis of this result, we discuss the possible use of the AdCAG-Cre vector to manipulate the gene expression in mammalian cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviruses, Human / genetics*
  • Animals
  • Base Sequence
  • Cells, Cultured
  • DNA Nucleotidyltransferases / genetics
  • DNA Primers / chemistry
  • Defective Viruses
  • Gene Expression Regulation, Viral
  • Gene Transfer Techniques
  • Genetic Vectors*
  • Humans
  • Integrases*
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Viral Proteins*


  • DNA Primers
  • Viral Proteins
  • Cre recombinase
  • DNA Nucleotidyltransferases
  • Integrases