Elevated calcium level induces calcium-dependent proteolysis of A-CAM (N-cadherin) in heart--analysis by detergent-treated model

Biochem Biophys Res Commun. 1995 Dec 14;217(2):649-53. doi: 10.1006/bbrc.1995.2823.


Calcium overload induces cardiac muscle cell dysfunction in cardiovascular diseases. We investigated the effects of elevated calcium level on adherens-junction-specific cell adhesion molecule (A-CAM). Incubation of Triton X-100-treated canine heart homogenate in the presence of Ca2+ reduced the content of A-CAM. Reduction in A-CAM requires milli-molar Ca2+ and was inhibited by protease inhibitors, leupeptin and calpeptin. Immunohistochemical observation revealed that m-calcium-activated neutral protease (m-CANP) was colocalized with A-CAM in intercalated disks. These data suggested that m-CANP proteolyzes A-CAM in response to calcium overload in cardiac muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cadherins / metabolism*
  • Calcium / physiology*
  • Calpain / metabolism
  • Cardiovascular Diseases / metabolism
  • Dogs
  • Fluorescent Antibody Technique, Indirect
  • Myocardium / metabolism*
  • Octoxynol / chemistry


  • Cadherins
  • Octoxynol
  • Calpain
  • Calcium