Characterization of the genomic sequence of type V (or 3a) hepatitis C virus isolates and PCR primers for specific detection

J Gen Virol. 1993 Nov;74 ( Pt 11):2385-90. doi: 10.1099/0022-1317-74-11-2385.


We have identified four new hepatitis C virus (HCV) isolates whose genomic RNA could be amplified by PCR using primers from the 5' untranslated region (UTR), but the RNA could not be detected with genotype I to IV (or types 1a, 1b, 2a and 2b respectively)-specific core region-derived primers. We compared the nucleotide sequences of the new isolates from positions 65 to 1850 (3' end of 5' UTR, C, E1 and 5' end of E2/NS1) and 8276 to 9394 (3' end of NS5 and 3' UTR) with those for genotypes I to IV. The four isolates had the following characteristics: (i) the overall nucleotide sequence similarity between the four isolates was 95 to 96%, compared to 73 to 74%, 73%, 70% or 69 to 70% against genotypes I, II, III or IV, respectively; (ii) the sequence similarity to other reported 'type V (3a)' isolates was 88 to 100%; (iii) the hypervariable region 1 [(HVR)-1] was present but HVR-2 was absent within the E2/NS1 region; (iv) only one in-frame termination codon was present for the presumed polyprotein; (v) the 3'UTR preceding a terminal poly(U) stretch was significantly shorter than in genotype I to IV isolates. We classified the four isolates as genotype V (3a), and searched for uniquely conserved nucleotide sequences that could be used for type-specific PCR. A core region-derived primer pair (no. 104V: 5' CGTAAAACTTCT GAACGGTC, sense and no. 339: 5' GCTGAGCCCA GGACCGGTCT, antisense) was identified and successfully used to diagnose genotype V (3a) HCV infection.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Complementary
  • Hepacivirus / classification
  • Hepacivirus / genetics*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA
  • RNA, Viral / genetics*


  • DNA, Complementary
  • RNA primers
  • RNA, Viral
  • RNA

Associated data

  • GENBANK/D14305
  • GENBANK/D14306
  • GENBANK/D14307
  • GENBANK/D14308
  • GENBANK/D14309
  • GENBANK/D14310
  • GENBANK/D14311
  • GENBANK/D14312